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High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)
Kapitel
- 00:05Introduction
- 00:21Antibody Incubation with Beads
- 01:35Chromatin Immunoprecipitation (ChIP)
- 02:56Enzymatic Reactions on Beads
- 04:59Elution and Purification
- 05:43Library Preparation
- 06:50Results: ChIP-exo Produces High Mapping Resolution
- 08:04Conclusion
Precise determination of protein-binding locations across the genome is important for understanding gene regulation. Here we describe a genomic mapping method that treats chromatin-immunoprecipitated DNA with exonuclease digestion (ChIP-exo) followed by high-throughput sequencing. This method detects protein-DNA interactions with near base-pair mapping resolution and high signal-to-noise ratio in mammalian neurons.
