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使用硫代巴比妥酸反应物质测定法评估生物样品中的氧化应激
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JoVE Journal
Chemistry
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Please note that all translations are automatically generated.
Click here for the English version.
使用硫代巴比妥酸反应物质测定法评估生物样品中的氧化应激
DOI:
10.3791/61122-v
•
06:19 min
•
May 12, 2020
•
Jesús Aguilar Diaz De Leon
,
Chad R. Borges*
1
School of Molecular Sciences, The Biodesign Institute – Center for Personalized Diagnostics
,
Arizona State University
Chapters
00:04
Introduction
01:08
Reagent Preparation
02:26
TBARS Assay
03:56
Results: Thiobarbituric Acid Reactive Substances Assay
05:30
Conclusion
Summary
Automatic Translation
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Automatic Translation
硫代巴比妥酸反应物质测定的目的是通过使用可见光波长分光光度法在532nm处测量脂质过氧化产物（主要是丙二醛）的产生来评估生物样品中的氧化应激。这里描述的方法可应用于人血清、细胞裂解物和低密度脂蛋白。
Tags
TBARS Assay
Oxidative Stress
Biological Samples
MDA
Thiobarbituric Acid
Sodium Hydroxide
Sodium Acetate
Spectrophotometric Analysis
Sample Preparation
Standard Curve
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