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JoVE Journal Chemistry
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
 

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

Article DOI: 10.3791/61122-v • 06:19 min • May 12th, 2020
May 12th, 2020 •
1,
1School of Molecular Sciences, The Biodesign Institute - Center for Personalized Diagnostics, Arizona State University

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Summary
Automatic Translation

The goal of the thiobarbituric acid reactive substances assay is to assess oxidative stress in biological samples by measuring the production of lipid peroxidation products, primarily malondialdehyde, using visible wavelength spectrophotometry at 532 nm. The method described here can be applied to human serum, cell lysates, and low density lipoproteins.

Tags

TBARS Assay Oxidative Stress Evaluation Biological Samples TBARS Levels Intra-laboratory Assessment Cost-effective Commercially Available Kits Batch Analysis Biospecimens Stability Studies Sample Preservation Fresh Reagents PH Control Absorbance Measurement Thiobarbituric Acid Solution Sodium Hydroxide Solution
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