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Potato Virus X-Based microRNA Silencing (VbMS) in Kartoffeln.
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Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
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Potato Virus X-Based microRNA Silencing (VbMS) in Kartoffeln.

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2,997 Views

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11:51 min

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May 11, 2020

DOI:

10.3791/61067-v

DOI:
10.3791/61067-v

•

11:51 min
May 11, 2020

•

13 Views
, ,
1Texas A&M AgriLife Research Center at Dallas,Texas A&M University System, 2Department of Plant Pathology, Microbiology,Texas A&M University

Summary

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Wir präsentieren ein detailliertes Protokoll für das auf Kartoffelvirus X (PVX) basierende microRNA-Silencing-System (VbMS) zur funktionellen Charakterisierung endogener microRNAs (miRNAs) in Kartoffeln. Target Mimic (TM) Moleküle von miRNA von Interesse werden in den PVX-Vektor integriert und vorübergehend in Kartoffeln exprimiert, um die Ziel-miRNA oder miRNA-Familie zum Schweigen zu bringen.

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Cite this Article

Zhao, J., Rios, C. G., Song, J. Potato Virus X-Based microRNA Silencing (VbMS) In Potato.. J. Vis. Exp. (159), e61067, doi:10.3791/61067 (2020).

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