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Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
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环境科学
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JoVE 杂志 环境科学
Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

2,997 Views

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11:51 min

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May 11, 2020

DOI:

10.3791/61067-v

DOI:
10.3791/61067-v

•

11:51 min
May 11, 2020

•

13 Views
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1Texas A&M AgriLife Research Center at Dallas,Texas A&M University System, 2Department of Plant Pathology, Microbiology,Texas A&M University

Summary

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We present a detailed protocol for potato virus X (PVX)-based microRNA silencing (VbMS) system to functionally characterize endogenous microRNAs (miRNAs) in potato. Target mimic (TM) molecules of miRNA of interest are integrated into the PVX vector and transiently expressed in potato to silence the target miRNA or miRNA family.

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Cite this Article

Zhao, J., Rios, C. G., Song, J. Potato Virus X-Based microRNA Silencing (VbMS) In Potato.. J. Vis. Exp. (159), e61067, doi:10.3791/61067 (2020).

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