A bottleneck in the ‘design-build-test’ cycle of microbial engineering is the speed at which we can perform functional screens of strains. We describe a high-throughput method for strain screening applied to hundreds to thousands of yeast cells per experiment that utilizes droplet-based RNA sequencing.
Zhang, J. Q., Chang, K., Liu, L., Gartner, Z. J., Abate, A. R. High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing. J. Vis. Exp. (159), e61014, doi:10.3791/61014 (2020).