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High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
JoVE Journal
Bioengenharia
This content is Free Access.
JoVE Journal Bioengenharia
High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

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07:55 min

May 21, 2020

DOI:

07:55 min
May 21, 2020

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Summary

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A bottleneck in the ‘design-build-test’ cycle of microbial engineering is the speed at which we can perform functional screens of strains. We describe a high-throughput method for strain screening applied to hundreds to thousands of yeast cells per experiment that utilizes droplet-based RNA sequencing.

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