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通过宫内注射诱导利普托门宁皮细胞修饰
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Induction of Leptomeningeal Cells Modification Via Intracisternal Injection
Please note that all translations are automatically generated.
Click here for the English version.
通过宫内注射诱导利普托门宁皮细胞修饰
DOI:
10.3791/61009-v
•
05:55 min
•
May 07, 2020
•
Margherita Zamboni
,
Giuseppe Santopolo
,
Jonas Frisén
1
Department of Cell and Molecular Biology
,
Karolinska Institute
Chapters
00:04
Introduction
01:07
Preparation of the Injection System
02:54
Intracisternal Injection
03:55
Results: Specific Labelling of Leptomeningeal Cells via Intracisternal Injection of Endoxifen
05:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种宫内注射,在头尖弯曲的针头可以稳定在头骨上,从而消除了对底层腹腔损伤的风险。该方法可用于脑膜蛋白酶细胞的基因命运图谱和操作,以及跟踪脑脊液运动。
Tags
Leptomeningeal Cells
Intracisternal Injection
Gene Editing
Neurodevelopment
Bacterial Meningitis
Cerebrospinal Fluid Flow
Hamilton Syringe
Endoxifen
Isoflurane
Cisterna Magna
Dural Membrane
Surgical Procedure
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