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Induction of Leptomeningeal Cells Modification Via Intracisternal Injection
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Induction of Leptomeningeal Cells Modification Via Intracisternal Injection
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May 07, 2020
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We describe an intracisternal injection that employs a needle bent at the tip that can be stabilized to the skull, thus eliminating the risk of damage to the underlying parenchyma. The approach can be used for genetic fate mapping and manipulations of leptomeningeal cells and for tracking cerebrospinal fluid movement.
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Zamboni, M., Santopolo, G., Frisén, J. Induction of Leptomeningeal Cells Modification Via Intracisternal Injection. J. Vis. Exp. (159), e61009, doi:10.3791/61009 (2020).
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