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脑切片和原细胞培养中金字塔神经元的弹道标记
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Neuroscience
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JoVE Journal Neuroscience
Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture
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脑切片和原细胞培养中金字塔神经元的弹道标记

DOI:
10.3791/60989-v

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09:40 min

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April 02, 2020

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, , ,
1Program in Behavioral Neuroscience, Department of Psychology,University of South Carolina

Chapters

  • 00:04Introduction
  • 00:38Preparation of Dil/Tungsten Bead Tubing
  • 02:55Preparation of Brain Sections
  • 04:20Ballistic Labeling and Visualization of Brain Sections
  • 06:16Cell Culture Method
  • 07:12Results: Analysis of Pyramidal Neurons Using Ballistic Labeling Technology
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出了一种用于标记和分析金字塔神经元的协议，这对于评估神经元和树突脊柱的潜在形态变化至关重要，这些变化可能是神经化学和行为异常的基础。

Tags

Ballistic LabelingPyramidal NeuronsBrain SlicesPrimary Cell CulturePVP SolutionTungsten Microcarrier BeadsLipophilic DiIC18(3) DyeMethylene ChlorideSonicationNitrogen Gas DryingRat Brain PerfusionPBSPFA

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