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Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells

Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells

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11:17 min

May 20, 2020

DOI:

11:17 min
May 20, 2020

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Summary

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Laser ablation is a widely applicable technology for studying regeneration in biological systems. The presented protocol describes use of a standard laser-scanning confocal microscope for laser ablation and subsequent time-lapse imaging of regenerating interneuromast cells in the zebrafish lateral line.

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