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Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells
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Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells
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May 20, 2020
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Laser ablation is a widely applicable technology for studying regeneration in biological systems. The presented protocol describes use of a standard laser-scanning confocal microscope for laser ablation and subsequent time-lapse imaging of regenerating interneuromast cells in the zebrafish lateral line.
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Volpe, B. A., Fotino, T. H., Steiner, A. B. Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells. J. Vis. Exp. (159), e60966, doi:10.3791/60966 (2020).
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