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Geração de Oligodendrocytes e Oligodendrocyte-Conditioned Medium para Experimentos de Cocultura
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Neuroscience
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JoVE Journal Neuroscience
Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments

Geração de Oligodendrocytes e Oligodendrocyte-Conditioned Medium para Experimentos de Cocultura

português

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10,028 Views

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09:05 min

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February 09, 2020

DOI:

10.3791/60912-v

DOI:
10.3791/60912-v

•

09:05 min
February 09, 2020

•

3 Views
, , , , ,
1Sorbonne Université, Inserm, CNRS, UMR7225,Institut du Cerveau et de la Moelle épinière, ICM, 2Assistance Publique-Hôpitaux de Paris,GH Pitié Salpêtrière, 3Ecole Supérieure des Techniques de Biologie Appliquée

Summary

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Aqui, apresentamos um método eficiente para a purificação de oligodendrocytes e produção de meio condicioso oligodendrocyte que pode ser usado para experimentos de cocultura.

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Cite this Article

Mazuir, E., Dubessy, A., Wallon, L., Aigrot, M., Lubetzki, C., Sol-Foulon, N. Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments. J. Vis. Exp. (156), e60912, doi:10.3791/60912 (2020).

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