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Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments
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JoVE Journal 神経科学
Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments

Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments

10,032 Views

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09:05 min

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February 09, 2020

DOI:

10.3791/60912-v

DOI:
10.3791/60912-v

•

09:05 min
February 09, 2020

•

3 Views
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1Sorbonne Université, Inserm, CNRS, UMR7225,Institut du Cerveau et de la Moelle épinière, ICM, 2Assistance Publique-Hôpitaux de Paris,GH Pitié Salpêtrière, 3Ecole Supérieure des Techniques de Biologie Appliquée

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Herein, we display an efficient method for the purification of oligodendrocytes and production of oligodendrocyte-conditioned medium that can be used for co-culture experiments.

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Mazuir, E., Dubessy, A., Wallon, L., Aigrot, M., Lubetzki, C., Sol-Foulon, N. Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments. J. Vis. Exp. (156), e60912, doi:10.3791/60912 (2020).

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