Presented here is a stepwise procedure for in vitro differentiation of human primary keratinocytes by contact inhibition followed by characterization at the molecular level by RNA-seq analysis.
Smits, J. G., Qu, J., Niehues, H., Zhou, H. Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis. J. Vis. Exp. (159), e60905, doi:10.3791/60905 (2020).