Rivista
/
Genetica
/
Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
JoVE Journal
Genetica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

DOI:
10.3791/60905-v

•

07:29 min

•

May 16, 2020

•

, , ,
1Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences,Radboud University, Nijmegen, The Netherlands, 2Department of Dermatology, Radboud University Medical Center,Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands, 3Department of Human Genetics,Radboud University Medical Center, Nijmegen, The Netherlands

Capitoli

  • 00:04Introduction
  • 01:10Human Primary Keratinocyte Differentiation by Contact Inhibition
  • 02:20RNA-seq Data Analysis
  • 04:53Results: Keratinocyte Differentiation and Analysis
  • 06:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

Presented here is a stepwise procedure for in vitro differentiation of human primary keratinocytes by contact inhibition followed by characterization at the molecular level by RNA-seq analysis.

Tags

In VitroKeratinocyte DifferentiationRNA-seq2D MonolayerProliferation MediumDifferentiation MediumCell Seeding DensityRNA IsolationCDNA ConversionLibrary PreparationNext-generation SequencingRead MappingDESeq2RLD NormalizationVST Normalization

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Differentiation, Maintenance, and Analysis of Human Retinal Pigment Epithelium Cells: A Disease-in-a-dish Model for BEST1 Mutations

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Improving Small RNA-seq: Less Bias and Better Detection of 2′-O-Methyl RNAs

Read Article