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微挥和荧光素-提拉美化物，用于小鼠肾上腺的多重免疫染色 – 使用来自同一宿主物种的未结合原抗体
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Developmental Biology
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JoVE Journal Developmental Biology
Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species
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微挥和荧光素-提拉美化物，用于小鼠肾上腺的多重免疫染色 – 使用来自同一宿主物种的未结合原抗体

English

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6,699 Views

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07:37 min

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February 21, 2020

DOI:

10.3791/60868-v

DOI:
10.3791/60868-v

•

07:37 min
February 21, 2020

•

2 Views
, , ,
1College of Animal Science & Technology,Henan University of Science and Technology, 2Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine,Auburn University

Summary

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利用来自同一宿主物种的初级抗体，已经建立了几种不同的多路免疫染色方法。在这里，我们描述了在正式固定石蜡嵌入小鼠肾上腺部分进行多倍免疫染色期间，使用微波介导的抗体剥离和荧光素-酪酰胺来阻止抗体的交叉反应。

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Cite this Article

Lyu, Q., Zheng, H. S., Laprocina, K., Huang, C. J. Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species. J. Vis. Exp. (156), e60868, doi:10.3791/60868 (2020).

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