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Hedefsiz Sıvı Kromatografisi-Kütle Spektrometresi Tabanlı Metabolomik Buğday Tanesi Analizi
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March 13, 2020
DOI:
1Research and Innovation,Murdoch University, 2Centre for Digital Agriculture,Curtin University, 3Centre for Integrative Metabolomics and Computational Biology, School of Science,Edith Cowan University, 4Centre for Computational and System Medicine, Health Futures Institute,Murdoch University, 5Department of Primary Industries and Regional Development, 6State Agricultural Biotechnology Centre,Murdoch University
Summary
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Buğday tanemetabolitleri ve lipitlerinin hedeflenmemiş analizi için bir yöntem sunulmuştur. Protokol, pozitif ve negatif elektrosprey iyonizasyon modlarında edinim ile bir asetonnril metabolit çıkarma yöntemi ve ters faz sıvı kromatografi-kütle spektrometresi metodolojisi içerir.
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Cite this Article
Abbiss, H., Gummer, J. P. A., Francki, M., Trengove, R. D. Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain. J. Vis. Exp. (157), e60851, doi:10.3791/60851 (2020).
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