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March 13, 2020
DOI:
1Research and Innovation,Murdoch University, 2Centre for Digital Agriculture,Curtin University, 3Centre for Integrative Metabolomics and Computational Biology, School of Science,Edith Cowan University, 4Centre for Computational and System Medicine, Health Futures Institute,Murdoch University, 5Department of Primary Industries and Regional Development, 6State Agricultural Biotechnology Centre,Murdoch University
Summary
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Apresenta-se um método para a análise não direcionada de metabólitos e lipídios de grãos de trigo. O protocolo inclui um método de extração metabólica acetonitrila e metodologia de espectrometria de cromatografia líquida de fase invertida, com aquisição em modos positivos e negativos de ionização eletrospray.
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Cite this Article
Abbiss, H., Gummer, J. P. A., Francki, M., Trengove, R. D. Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain. J. Vis. Exp. (157), e60851, doi:10.3791/60851 (2020).
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