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使用头太空气色谱对斑马鱼胚胎中的乙醇水平进行定量
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography
DOI:

08:22 min

February 11, 2020

Chapters

  • 00:04Introduction
  • 00:58Measuring the Embryonic Volume Using Water Displacement
  • 02:14Treating Embryos with Ethanol
  • 02:50Processing Embryos for Head Space Gas Chromatography
  • 03:58Sample Measurements Using Gas Space Chromatography
  • 05:04Sample Ethanol Peak Integration and Concentration Analysis
  • 06:06Results: Ethanol Levels in Early Embryonic Zebrafish
  • 07:38Conclusion

Summary

Automatic Translation

这项工作描述了一种使用头部空间气相色谱从适当的暴露方法到胚胎加工和乙醇分析来量化斑马鱼胚胎中乙醇水平的协议。

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