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Kalıtsal Spastik Paraplejide İnsan Kaynaklı Pluripotent Kök Hücre Kaynaklı Nöronlarda Mitokondriyal Taşıma ve Morfolojinin Analizi
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Neuroscience
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JoVE Journal Neuroscience
Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia
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Kalıtsal Spastik Paraplejide İnsan Kaynaklı Pluripotent Kök Hücre Kaynaklı Nöronlarda Mitokondriyal Taşıma ve Morfolojinin Analizi

Türkçe

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7,562 Views

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07:32 min

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February 09, 2020

DOI:

10.3791/60548-v

DOI:
10.3791/60548-v

•

07:32 min
February 09, 2020

•

7 Views
, , , ,
1Department of Biomedical Sciences,University of Illinois College of Medicine Rockford, 2Department of Bioengineering,University of Illinois at Chicago, 3MD Program,University of Illinois College of Medicine Rockford

Summary

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Bozulmuş mitokondriyal taşıma ve morfolojisi çeşitli nörodejeneratif hastalıklarda rol oynamaktadır. Sunulan protokol, kalıtsal spastik paraplejide mitokondriyal taşıma ve morfolojiyi değerlendirmek için indüklenmiş pluripotent kök hücre kaynaklı ön beyin nöronlarını kullanır. Bu protokol, aksonların boyunca mitokondriyal ticaretinin karakterizasyonuna ve nörodejeneratif hastalıkların incelenmesini kolaylaştıracak morfolojilerinin analizine olanak sağlamaktadır.

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Cite this Article

Mou, Y., Mukte, S., Chai, E., Dein, J., Li, X. Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia. J. Vis. Exp. (156), e60548, doi:10.3791/60548 (2020).

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