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Analisi del trasporto mitocondriale e della morfologia nei neuroni derivati dalle cellule staminali indotte dall'uomo in paraplegia ereditaria
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Neuroscience
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JoVE Journal Neuroscience
Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia
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Analisi del trasporto mitocondriale e della morfologia nei neuroni derivati dalle cellule staminali indotte dall'uomo in paraplegia ereditaria

italiano

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7,537 Views

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07:32 min

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February 09, 2020

DOI:

10.3791/60548-v

DOI:
10.3791/60548-v

•

07:32 min
February 09, 2020

•

7 Views
, , , ,
1Department of Biomedical Sciences,University of Illinois College of Medicine Rockford, 2Department of Bioengineering,University of Illinois at Chicago, 3MD Program,University of Illinois College of Medicine Rockford

Summary

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Il trasporto mitocondriale alterato e la morfologia sono coinvolti in varie malattie neurodegenerative. Il protocollo presentato utilizza neuroni del prosencefalo derivati da cellule staminali pluripotenti indotte per valutare il trasporto mitocondriale e la morfologia nella paraplegia spastica ereditaria. Questo protocollo consente la caratterizzazione del traffico mitocondriale lungo gli assoni e l'analisi della loro morfologia, che faciliterà lo studio delle malattie neurodegenerative.

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Mou, Y., Mukte, S., Chai, E., Dein, J., Li, X. Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia. J. Vis. Exp. (156), e60548, doi:10.3791/60548 (2020).

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