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Quantitative Measurement of Intrathecally Synthesized Proteins in Mice
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Neuroscience
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JoVE Journal Neuroscience
Quantitative Measurement of Intrathecally Synthesized Proteins in Mice

Quantitative Measurement of Intrathecally Synthesized Proteins in Mice

English

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10,598 Views

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08:23 min

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November 29, 2019

DOI:

10.3791/60495-v

DOI:
10.3791/60495-v

•

08:23 min
November 29, 2019

•

9 Views
, , , , ,
1Department of Neurology,Geisel School of Medicine & Dartmouth-Hitchcock Medical Center, 2Program in Experimental and Molecular Medicine,Dartmouth College

Summary

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Elevated spinal fluid protein levels can either be the result of diffusion of plasma protein across an altered blood-brain barrier or intrathecal synthesis. An optimized testing protocol is presented in this article that helps to discriminate both cases and provides quantitative measurements of intrathecally synthesized proteins.

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Cite this Article

Gilli, F., Welsh, N. C., Linzey, M. R., Royce, D. B., DiSano, K. D., Pachner, A. R. Quantitative Measurement of Intrathecally Synthesized Proteins in Mice. J. Vis. Exp. (153), e60495, doi:10.3791/60495 (2019).

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