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October 25, 2019
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Bacterial pathogens secrete proteins into the host that target crucial biological processes. Identifying the host pathways targeted by bacterial effector proteins is key to addressing molecular pathogenesis. Here, a method using a modified yeast suppressor and toxicity screen to elucidate host pathways targeted by toxic bacterial effector proteins is described.
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Yeast Toxicity ScreenYeast Suppressor ScreenBacterial Effector ProteinsHost PathwaysChlamydia TrachomatisLegionella PneumophilaCoxiella BurnetiiIntracellular PathogensProtein-protein InteractionsToxicity AssayGrowth AssayPlasmid TransformationDilution SeriesGalactose InductionYeast CultureCentrifugationLithium Acetate
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Faris, R., Weber, M. M. Identification of Host Pathways Targeted by Bacterial Effector Proteins using Yeast Toxicity and Suppressor Screens. J. Vis. Exp. (152), e60488, doi:10.3791/60488 (2019).
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