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Subcellulaire fractionering van primaire chronische lymfatische leukemie cellen om te controleren van nucleaire/cytoplasmatische eiwit handel
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking
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Subcellulaire fractionering van primaire chronische lymfatische leukemie cellen om te controleren van nucleaire/cytoplasmatische eiwit handel

Nederlands

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10,901 Views

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11:39 min

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October 23, 2019

DOI:

10.3791/60426-v

DOI:
10.3791/60426-v

•

11:39 min
October 23, 2019

•

3 Views
, , ,
1Paul O’Gorman Leukaemia Research Centre, Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences,University of Glasgow

Summary

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Dit protocol maakt de optimalisatie en de daaropvolgende efficiënte generatie van nucleaire en cytoplasmatische fracties van primaire chronische lymfatische leukemie cellen. Deze monsters worden gebruikt om te bepalen van de eiwit lokalisatie, alsmede veranderingen in de handel in eiwitten die plaatsvinden tussen de nucleaire en cytoplasmische compartimenten op celstimulatie en medicamenteuze behandeling.

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Cite this Article

Hay, J., Moles, M. W., Cassels, J., Michie, A. M. Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking. J. Vis. Exp. (152), e60426, doi:10.3791/60426 (2019).

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