Journal
/
Cancer Research
/
Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking

English

Automatically Generated

10,901 Views

•

11:39 min

•

October 23, 2019

DOI:

10.3791/60426-v

DOI:
10.3791/60426-v

•

11:39 min
October 23, 2019

•

3 Views
, , ,
1Paul O’Gorman Leukaemia Research Centre, Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences,University of Glasgow

Summary

Automatically generated

This protocol enables the optimization and subsequent efficient generation of nuclear and cytoplasmic fractions from primary chronic lymphocytic leukemia cells. These samples are used to determine protein localization as well as changes in protein trafficking that take place between the nuclear and cytoplasmic compartments upon cell stimulation and drug treatment.

Explore More Videos

Subcellular FractionationChronic Lymphocytic LeukemiaNuclear/cytoplasmic Protein TraffickingTumor Suppressor ProteinsPathogenesisB Cell MalignanciesProtein LocalizationProtein MovementDensity Gradient CentrifugationMononuclear Cells

Read Article

Cite this Article

Hay, J., Moles, M. W., Cassels, J., Michie, A. M. Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking. J. Vis. Exp. (152), e60426, doi:10.3791/60426 (2019).

Download .ris file

Copy

Share Video

.

Copy