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Developmental Biology
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使用纸屑小鼠研究矿化组织的克隆遗传追踪
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Clonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues
Please note that all translations are automatically generated.
Click here for the English version.
使用纸屑小鼠研究矿化组织的克隆遗传追踪
DOI:
10.3791/60424-v
•
07:41 min
•
October 23, 2019
•
Baoyi Zhou
,
Marketa Kaucka
,
Andrei S. Chagin
2
,
Phillip T. Newton
3
1
Department of Physiology and Pharmacology
,
Karolinska Institutet
,
2
Institute for Regenerative Medicine
,
Sechenov First Moscow State Medical University (Sechenov University)
,
3
Department of Women’s and Children’s Health, Karolinska Institutet and Pediatric Endocrinology Unit
,
Karolinska University Hospital
Chapters
00:04
Title
01:07
Tissue Fixation and Processing
02:50
Sectioning and Slide Preparation
04:09
Confocal Microscopy
05:42
Results: Confetti Images from a Col2CreERT: Confetti Mouse
06:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方法描述了使用R26R-纸屑(纸屑)小鼠模型来研究矿化组织,涵盖了从育种策略到图像获取的所有步骤。包括一个可应用于所有软组织的一般协议和可应用于矿化组织的改良协议。
Tags
Clonal Genetic Tracing
Confetti Mouse
Fluorescent Proteins
Regenerative Medicine
Developmental Biology
Formaldehyde
EDTA
Sucrose
Cryosectioning
OCT Compound
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