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Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers
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Genetics
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JoVE Journal Genetics
Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

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7,575 Views

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10:28 min

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December 06, 2019

DOI:

10.3791/60413-v

DOI:
10.3791/60413-v

•

10:28 min
December 06, 2019

•

2 Views
, , ,
1Department of Cell Biology,Yale School of Medicine, 2Department of Biological Sciences,University of North Carolina at Charlotte

Summary

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Here, we present a workflow for the expression, purification and liposome binding of SNX-BAR heterodimers in yeast.

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SNX-BARMembrane RemodelingLiposome BindingYeast ExpressionProtein PurificationIgG Sepharose BeadsCell LysisCentrifugationExtrusionBiophysical PropertiesLipid Specificity

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Ma, M., Goyal, S., Burd, C. G., Chi, R. J. Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers. J. Vis. Exp. (154), e60413, doi:10.3791/60413 (2019).

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