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Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers
JoVE Journal
Genetik
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JoVE Journal Genetik
Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

DOI:
10.3791/60413-v

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10:28 min

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December 06, 2019

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, , ,
1Department of Cell Biology,Yale School of Medicine, 2Department of Biological Sciences,University of North Carolina at Charlotte

Bölümler

  • 00:04Başlık
  • 00:58Yeast Induction and Sorting Nexin-Bin-Amphiphysin-Rvs (SNX-BAR) Dimer Purification
  • 03:52Liposome Preparation
  • 06:44SNX-BAR Liposome Binding and Tubulation
  • 08:26Results: Representative SNX-BAR Induction Verification, Purification, and Liposome Binding and Tubulation Assays
  • 09:38Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we present a workflow for the expression, purification and liposome binding of SNX-BAR heterodimers in yeast.

Etiketler

SNX-BARMembrane RemodelingLiposome BindingYeast ExpressionProtein PurificationIgG Sepharose BeadsCell LysisCentrifugationExtrusionBiophysical PropertiesLipid Specificity

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