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Microsatelliet DNA genotypering en flow Cytometrie ploidy analyses van Formalin-vaste paraffine-ingesloten Hydatidiforme molaire weefsels
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Genetics
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JoVE Journal Genetics
Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
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Microsatelliet DNA genotypering en flow Cytometrie ploidy analyses van Formalin-vaste paraffine-ingesloten Hydatidiforme molaire weefsels

Nederlands

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8,996 Views

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11:54 min

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October 20, 2019

DOI:

10.3791/60366-v

DOI:
10.3791/60366-v

•

11:54 min
October 20, 2019

•

6 Views
, , ,
1Department of Experimental Medicine,McGill University, 2Department of Human Genetics,McGill University, 3Department of Obstetrics and Gynecology,McGill University

Summary

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Hydatidiforme mollen zijn abnormale humane zwangerschappen met heterogene etiologieën die kunnen worden geclassificeerd op basis van hun morfologische kenmerken en de ouderlijke bijdrage aan de molaire Genomes. Hier worden protocollen van multiplex microsatelliet DNA-genotypering en Flowcytometrie van formalin-Fixed paraffine-ingesloten molaire weefsels gedetailleerd beschreven, samen met de interpretatie en integratie van resultaten.

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Cite this Article

Khawajkie, Y., Mechtouf, N., Nguyen, P., Slim, R. Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues. J. Vis. Exp. (152), e60366, doi:10.3791/60366 (2019).

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