Journal
/
Genética
/
Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

8,996 Views

•

11:54 min

•

October 20, 2019

DOI:

10.3791/60366-v

DOI:
10.3791/60366-v

•

11:54 min
October 20, 2019

•

6 Views
, , ,
1Department of Experimental Medicine,McGill University, 2Department of Human Genetics,McGill University, 3Department of Obstetrics and Gynecology,McGill University

Summary

Automatically generated

Hydatidiform moles are abnormal human pregnancies with heterogeneous aetiologies that can be classified according to their morphological features and parental contribution to the molar genomes. Here, protocols of multiplex microsatellite DNA genotyping and flow cytometry of formalin-fixed paraffin-embedded molar tissues are described in detail, together with results’ interpretation and integration.

Explore More Videos

Microsatellite DNA GenotypingFlow CytometryPloidy AnalysisFormalin-fixed Paraffin-embeddedContaminationMaternal DNADiagnosisH&E StainingChorionic VilliDNA Extraction

Read Article

Cite this Article

Khawajkie, Y., Mechtouf, N., Nguyen, P., Slim, R. Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues. J. Vis. Exp. (152), e60366, doi:10.3791/60366 (2019).

Download .ris file

Copy

Share Video

.

Copy