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January 14, 2020
DOI:
1Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics,University of Houston, 2Institute of Biosciences and Technology,Texas A&M Health Science Center, 3Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling,University of Houston, 4Department of Biosciences and Nutrition,Novum, Karolinska Institutet, 5Department of Intelligent Systems Engineering,Indiana University
Summary
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本文介绍了一种将脆弱的斑马鱼胚胎用于延长延时共聚焦显微镜的方法。这种低成本的方法很容易使用常规玻璃底显微镜盘在任何倒置显微镜上成像。安装以不同浓度的甘蔗层进行。
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Cite this Article
Upadhyay, S., Vergara, L., Shah, P., Gustafsson, J., Kakadiaris, I., Bondesson, M. A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos. J. Vis. Exp. (155), e60321, doi:10.3791/60321 (2020).
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