/
/
A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
8,609 Views
•
08:55 min
•
January 14, 2020
DOI:
1Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics,University of Houston, 2Institute of Biosciences and Technology,Texas A&M Health Science Center, 3Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling,University of Houston, 4Department of Biosciences and Nutrition,Novum, Karolinska Institutet, 5Department of Intelligent Systems Engineering,Indiana University
Summary
Automatically generated
This article describes a method to mount fragile zebrafish embryos for extended time-lapse confocal microscopy. This low-cost method is easy to perform using regular glass-bottom microscopy dishes for imaging on any inverted microscope. The mounting is performed in layers of agarose at different concentrations.
Read Article
Cite this Article
Upadhyay, S., Vergara, L., Shah, P., Gustafsson, J., Kakadiaris, I., Bondesson, M. A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos. J. Vis. Exp. (155), e60321, doi:10.3791/60321 (2020).
Copy