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在五个脊椎动物群中可靠隔离中枢神经系统微血管
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Neuroscience
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JoVE Journal Neuroscience
Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups
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在五个脊椎动物群中可靠隔离中枢神经系统微血管

DOI:
10.3791/60291-v

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10:35 min

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January 12, 2020

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, , , ,
1Department of Anatomy, Physiology and Cell Biology,University of California Davis, 2University of Veterinary Medicine Hannover Foundation

Chapters

  • 00:04Title
  • 00:40Central Nervous System (CNS) Tissue Dissection
  • 02:35CNS Tissue Homogenization
  • 04:20Microvessel Purification
  • 05:57Microvessel Elution and Filtration
  • 07:44Results: Representative Microvessel Protein Expression
  • 09:57Conclusion

Summary

Automatic Translation

Automatic Translation

该协议的目的是从脑血管和陀螺脊椎动物的中枢神经系统的多个区域分离微血管。

Tags

Microvessel IsolationCentral Nervous SystemVertebratesDissectionMeningesChoroid PlexusHomogenization

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