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Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System
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May 12, 2011
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Optic Nerve transection is a widely used model of adult CNS injury. Ninety percent of retinal ganglion cells (RGCs) whose axons are completely transected (axotomy) die within 14 days after axotomy. This model is easily amenable to experimental manipulations and highly reproducible.
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Optic Nerve TransectionAdult Neuron ApoptosisCentral Nervous SystemRetinal Ganglion CellsOptic NerveAxotomizedApoptotic Neuronal Cell DeathCNS ModelVitreous ChamberDrug DeliveryIntraocular InjectionsShort Interfering RNAs (siRNAs)PlasmidsViral VectorsSuperior ColliculusApoptotic MechanismsNeuronal PopulationCell Survival QuantificationFluorescent Tracer
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Magharious, M. M., D'Onofrio, P. M., Koeberle, P. D. Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System. J. Vis. Exp. (51), e2241, doi:10.3791/2241 (2011).
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