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Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis
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Neuroscience
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JoVE Journal Neuroscience
Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis

Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis

DOI:
10.3791/60225-v

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07:38 min

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September 14, 2019

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, , ,
1Department of Physiology, David Geffen School of Medicine,University of California Los Angeles, 2Department of Neurobiology, David Geffen School of Medicine,University of California Los Angeles

Chapters

  • 00:04Title
  • 00:31Electrode Preparation
  • 01:25DYE Ejection Test
  • 02:44Astrocyte Filling
  • 04:24Imaging of Filled Astrocyte
  • 05:15Results: Visualization of Astrocyte Morphology
  • 06:56Conclusion

Summary

Automatic Translation

Automatic Translation

Astrocytes are morphologically complex cells, exemplified by their multiple processes and bushy territories. To analyze their elaborate morphology, we present a reliable protocol to perform intracellular Lucifer yellow iontophoresis in lightly fixed tissue.

Tags

Keywords: Astrocyte MorphologyLucifer Yellow IontophoresisAstrocyte-neuronal InteractionsSynapseBrain SliceConfocal MicroscopyDye EjectionAstrocyte IdentificationStratum RadiatumHippocampus

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