Journal
/
Neuroscience
/
Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis

Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis

English

Automatically Generated

12,076 Views

•

07:38 min

•

September 14, 2019

DOI:

10.3791/60225-v

DOI:
10.3791/60225-v

•

07:38 min
September 14, 2019

•

35 Views
, , ,
1Department of Physiology, David Geffen School of Medicine,University of California Los Angeles, 2Department of Neurobiology, David Geffen School of Medicine,University of California Los Angeles

Summary

Automatically generated

Astrocytes are morphologically complex cells, exemplified by their multiple processes and bushy territories. To analyze their elaborate morphology, we present a reliable protocol to perform intracellular Lucifer yellow iontophoresis in lightly fixed tissue.

Explore More Videos

Astrocyte MorphologyLucifer Yellow IontophoresisAstrocyte-neuronal InteractionsSynapseBrain SliceConfocal MicroscopyDye EjectionAstrocyte IdentificationStratum RadiatumHippocampus

Read Article

Cite this Article

Moye, S. L., Diaz-Castro, B., Gangwani, M. R., Khakh, B. S. Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis. J. Vis. Exp. (151), e60225, doi:10.3791/60225 (2019).

Download .ris file

Copy

Share Video

.

Copy