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Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis
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JoVE Journal Neurowissenschaften
Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis

Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis

12,076 Views

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07:38 min

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September 14, 2019

DOI:

10.3791/60225-v

DOI:
10.3791/60225-v

•

07:38 min
September 14, 2019

•

35 Views
, , ,
1Department of Physiology, David Geffen School of Medicine,University of California Los Angeles, 2Department of Neurobiology, David Geffen School of Medicine,University of California Los Angeles

Summary

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Astrocytes are morphologically complex cells, exemplified by their multiple processes and bushy territories. To analyze their elaborate morphology, we present a reliable protocol to perform intracellular Lucifer yellow iontophoresis in lightly fixed tissue.

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Moye, S. L., Diaz-Castro, B., Gangwani, M. R., Khakh, B. S. Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis. J. Vis. Exp. (151), e60225, doi:10.3791/60225 (2019).

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