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Optisk billeddannelse af isolerede murine ventrikulære Myocytter
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Optical Imaging of Isolated Murine Ventricular Myocytes

Optisk billeddannelse af isolerede murine ventrikulære Myocytter

DOI:
10.3791/60196-v

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11:32 min

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January 17, 2020

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, , , , , , ,
1Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine,University of Science and Technology of China, Hefei, Anhui, China, 2Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, 3Pediatric Cardiac and Thoracic Surgery,University Hospitals Cleveland Medical Center

Chapters

  • 00:05Title
  • 01:26Preparation of Coverslips
  • 02:08Myocyte Isolation
  • 05:53Membrane Potential Dye Loading
  • 07:01Photometry and Charge Coupled Device Recordings
  • 09:21Results: Calcium and Sarcomere Shortening
  • 10:48Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer metoden til isolering af murine myocytter og hvordan man får spænding eller calcium spor samtidig med sarcomere afkortning af spor ved hjælp af fluorescens fotometri med samtidige digitale celle geometri målinger.

Tags

Optical ImagingMurine Ventricular MyocytesHeart FailureCardiac MetabolismExcitation-contraction CouplingCardiac Myocyte IsolationFluorescent ProbeCardiac ToxicityHeart Failure TherapeuticsTransgenic MiceLamininLangendorf PerfusionKHB-HB SolutionDigestion Buffer

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