/
/
In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen
This content is Free Access.
13,437 Views
•
07:54 min
•
September 20, 2012
DOI:
Summary
Automatically generated
Simple and reproducible procedures are described for making three structurally distinct collagen assemblies from a common commercially available Type I collagen monomer. Native type, fibrous long spacing or segmental long spacing collagen can be constructed by varying the conditions to which the 300 nm long and 1.4 nm diameter monomer building block is exposed.
Explore More Videos
In Vitro SynthesisNative CollagenFibrous Long Spacing CollagenSegmental Long Spacing CollagenExtracellular MatrixStructural ScaffoldingMechanical StrengthCollagen FibrilsBanding PeriodicityType I Collagen MonomersHierarchical AssemblyLength ScalesMicronsCommercially Available Collagen MonomerProtocolsMixtures Of StructuresUnbanded FibrilsImagingAtomic Force Microscope
Read Article
Cite this Article
Loo, R. W., Goh, J. B., Cheng, C. C., Su, N., Goh, M. C. In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen. J. Vis. Exp. (67), e4417, doi:10.3791/4417 (2012).
Copy