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siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells SiRNA Electroporation to Modulate Autophagie in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
DOI:

09:10 min

October 28, 2019

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Chapters

  • 00:04Title
  • 01:02Generation and Handling of Immature Dendritic Cells and Mature Dendritic Cells
  • 02:02Infection Procedure of DCs with HSV-1 and Interference of HSV-1-induced Autophagic Flux via Spautin-1 or Bafilomycin-A1
  • 04:17Interference of HSV-1-induced Autophagic Flux via Electroporation of iDCs Using FIP200-siRNA
  • 06:21Results: Analyses of Autophagic Flux in HSV-1-infected iDCs and mDCs
  • 08:10Conclusion

Summary

Automatic Translation

Dans cette étude, nous présentons des stratégies inhibitrices et siRNA-basées pour interférer avec le flux autophagique dans le virus simplex d'herpès type-1 (HSV-1)--infecté des cellules monocyte-dérivées de monocyte-dérivées.

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