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Aislamiento de poblaciones de neuronas específicas de Roundworm Caenorhabditis elegans
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans
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Aislamiento de poblaciones de neuronas específicas de Roundworm Caenorhabditis elegans

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6,137 Views

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09:42 min

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August 06, 2019

DOI:

10.3791/60145-v

DOI:
10.3791/60145-v

•

09:42 min
August 06, 2019

•

1 Views
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1Department of Biochemistry,University of Nebraska, 2Nebraska Redox Biology Center,University of Nebraska, 3Nebraska Center for Integrated Biomolecular Communication,University of Nebraska, 4Fred & Pamela Buffett Cancer Center,University of Nebraska Medical Center

Summary

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Aquí, presentamos un protocolo para el aislamiento simple de grupos específicos de células neuronales vivas que expresan proteína fluorescente verde de líneas transgénicas de Caenorhabditis elegans. Este método permite una variedad de estudios ex vivo centrados en neuronas específicas y tiene la capacidad de aislar las células para el cultivo a corto plazo.

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Germany, E. M., Zahayko, N., Khalimonchuk, O. Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans. J. Vis. Exp. (150), e60145, doi:10.3791/60145 (2019).

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