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Isolamento di popolazioni di neuroni specifici da nematode Caenorhabditis elegans
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans
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Isolamento di popolazioni di neuroni specifici da nematode Caenorhabditis elegans

italiano

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6,137 Views

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09:42 min

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August 06, 2019

DOI:

10.3791/60145-v

DOI:
10.3791/60145-v

•

09:42 min
August 06, 2019

•

1 Views
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1Department of Biochemistry,University of Nebraska, 2Nebraska Redox Biology Center,University of Nebraska, 3Nebraska Center for Integrated Biomolecular Communication,University of Nebraska, 4Fred & Pamela Buffett Cancer Center,University of Nebraska Medical Center

Summary

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Qui, presentiamo un protocollo per un semplice isolamento di specifici gruppi di cellule neuronali vive che esprimono proteine fluorescenti verdi dalle linee transgeniche di Caenorhabditis elegans . Questo metodo consente una varietà di studi ex vivo focalizzati su neuroni specifici e ha la capacità di isolare le cellule per ulteriori colture a breve termine.

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Cite this Article

Germany, E. M., Zahayko, N., Khalimonchuk, O. Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans. J. Vis. Exp. (150), e60145, doi:10.3791/60145 (2019).

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