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Adsorção molecular induzida por luz de proteínas usando o sistema PRIMO para Micropadronização para estudar as respostas celulares às proteínas da matriz extracelular
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Adsorção molecular induzida por luz de proteínas usando o sistema PRIMO para Micropadronização para estudar as respostas celulares às proteínas da matriz extracelular

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11,422 Views

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09:49 min

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October 11, 2019

DOI:

10.3791/60092-v

DOI:
10.3791/60092-v

•

09:49 min
October 11, 2019

•

32 Views
, , , , , ,
1Faculty of Biology, Medicine and Health. Division of Cell Matrix, Biology and Regenerative Medicine, Wellcome Trust Centre for Cell-Matrix Research,The University of Manchester, 2School of Materials,The University of Manchester, 3Blond McIndoe Laboratories, School of Biological Sciences, Faculty of Biology, Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester,Manchester Academic Health Science Centre, 4Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust,Manchester Academic Health Science Centre, 5Department of Pathology,UMC Utrecht

Summary

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Nosso objetivo geral é entender como as células sentem sinais extracelulares que levam ao crescimento axonal direcionado. Aqui, nós descrevemos a metodologia da adsorção molecular Light-induced das proteínas, usadas para produzir micropatterns definidos de componentes da matriz extracelular a fim estudar os eventos específicos que governam o conseqüência do AXON e o pathfinding.

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Cite this Article

Melero, C., Kolmogorova, A., Atherton, P., Derby, B., Reid, A., Jansen, K., Ballestrem, C. Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins. J. Vis. Exp. (152), e60092, doi:10.3791/60092 (2019).

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