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Lichtgeïnduceerde moleculaire adsorptie van eiwitten met behulp van het PRIMO-systeem voor micro patronen om Celresponsen te bestuderen op extracellulaire matrix eiwitten
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins
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Lichtgeïnduceerde moleculaire adsorptie van eiwitten met behulp van het PRIMO-systeem voor micro patronen om Celresponsen te bestuderen op extracellulaire matrix eiwitten

Nederlands

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11,422 Views

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09:49 min

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October 11, 2019

DOI:

10.3791/60092-v

DOI:
10.3791/60092-v

•

09:49 min
October 11, 2019

•

32 Views
, , , , , ,
1Faculty of Biology, Medicine and Health. Division of Cell Matrix, Biology and Regenerative Medicine, Wellcome Trust Centre for Cell-Matrix Research,The University of Manchester, 2School of Materials,The University of Manchester, 3Blond McIndoe Laboratories, School of Biological Sciences, Faculty of Biology, Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester,Manchester Academic Health Science Centre, 4Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust,Manchester Academic Health Science Centre, 5Department of Pathology,UMC Utrecht

Summary

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Ons algemene doel is om te begrijpen hoe cellen extracellulaire signalen voelen die leiden tot gerichte axonale groei. Hier beschrijven we de methodologie van licht-geïnduceerde moleculaire adsorptie van eiwitten, gebruikt om gedefinieerde micro-patronen van extracellulaire matrix componenten te produceren om specifieke gebeurtenissen te bestuderen die axon-uitgroei en pathfinding beheersen.

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Cite this Article

Melero, C., Kolmogorova, A., Atherton, P., Derby, B., Reid, A., Jansen, K., Ballestrem, C. Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins. J. Vis. Exp. (152), e60092, doi:10.3791/60092 (2019).

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