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הדור של שינויים גנומית מוגדרים באמצעות CRISPR-CAS9 בתאי גזע האדם Pluripotent

JoVE Journal
Genetics

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JoVE Journal Genetics

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

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הדור של שינויים גנומית מוגדרים באמצעות CRISPR-CAS9 בתאי גזע האדם Pluripotent

7,996 Views

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09:04 min

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September 25, 2019

DOI:

10.3791/60085-v

DOI:

10.3791/60085-v

•

09:04 min

September 25, 2019

•

5 Views

Fabian L. Cardenas-Diaz*¹, Jean Ann Maguire*¹, Paul Gadue^2,3, Deborah L. French^2,3

¹Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia, ²Department of Pathology and Laboratory Medicine,The Children’s Hospital of Philadelphia, ³Department of Pathology and Laboratory Medicine,University of Pennsylvania

Tags

CRISPR-Cas9 Human Pluripotent Stem Cells Isogenic Lines Heterozygous Mutations Gene Function Disease Modeling Colony Picking Fluorescence-activated Cell Sorting MEFs ROCK Inhibitor

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