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Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
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Genetik
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JoVE Journal Genetik
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

8,016 Views

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09:04 min

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September 25, 2019

DOI:

10.3791/60085-v

DOI:
10.3791/60085-v

•

09:04 min
September 25, 2019

•

3 Views
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1Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia, 2Department of Pathology and Laboratory Medicine,The Children’s Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine,University of Pennsylvania

Özet

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This protocol provides a method to facilitate the generation of defined heterozygous or homozygous nucleotide changes using CRISPR-CAS9 in human pluripotent stem cells.

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Cite this Article

Cardenas-Diaz, F. L., Maguire, J. A., Gadue, P., French, D. L. Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells. J. Vis. Exp. (151), e60085, doi:10.3791/60085 (2019).

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