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外迁移系统中淋巴细胞迁移的评估
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
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外迁移系统中淋巴细胞迁移的评估

English

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6,639 Views

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10:25 min

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September 20, 2019

DOI:

10.3791/60060-v

DOI:
10.3791/60060-v

•

10:25 min
September 20, 2019

•

1 Views
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1Department of Internal Medicine,University of Utah, 2Research Service,VA Nebraska Iowa Health Care System, 3Department of Internal Medicine,University of Nebraska Medical Center, 4Department of Environmental, Agricultural & Occupational Health,University of Nebraska Medical Center

Summary

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在此协议中，淋巴细胞被放置在迁移系统的顶室中，由多孔膜与底部腔室隔开。化学因子被添加到底部腔室，从而诱导沿化学基质梯度的活性迁移。48小时后，淋巴细胞在两个腔室计数，以定量迁移。

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Cite this Article

Warren, K. J., Wyatt, T. A. Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System. J. Vis. Exp. (151), e60060, doi:10.3791/60060 (2019).

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