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August 23, 2019
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Cell divisions can be visualized in real time using fluorescently tagged proteins and time-lapse microscopy. Using the protocol presented here, users can analyze cell division timing dynamics, mitotic spindle assembly, and chromosome congression and segregation. Defects in these events following RNA interference (RNAi)-mediated gene knockdown can be assessed and quantified.
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Dewey, E. B., Parra, A. S., Johnston, C. A. Use of Drosophila S2 Cells for Live Imaging of Cell Division. J. Vis. Exp. (150), e60049, doi:10.3791/60049 (2019).
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