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Test diagnostico immunocromatico rapido sulla rabbia sul campo per impostazioni limitate alle risorse con ulteriori applicazioni molecolari
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications
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Test diagnostico immunocromatico rapido sulla rabbia sul campo per impostazioni limitate alle risorse con ulteriori applicazioni molecolari

italiano

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12,643 Views

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07:40 min

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June 29, 2020

DOI:

10.3791/60008-v

DOI:
10.3791/60008-v

•

07:40 min
June 29, 2020

•

4 Views
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1Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies,Institut Pasteur, 2Environment and Sustainability Institute,University of Exeter, Penryn Campus, 3Institut de Recherche en Elevage pour le Développement, 4Laboratoire Central Vétérinaire, 5Direction des Services Vétérinaires, 6Ecole Inter Etats de Sciences et de Médecine Vétérinaires de Dakar, 7Laboratoire Central Vétérinaire de Bingerville,Laboratoire National d’Appui au Développement Agricole Bingerville, 8FAO Reference Centre for Rabies,Istituto Zooprofilattico Sperimentale delle Venezie, 9Swiss Tropical and Public Health Institute, 10University of Basel, 11Institut National de Recherche Biomédicale

Summary

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Presentiamo un protocollo completo per la diagnosi post-mortem della rabbia animale in condizioni di campo utilizzando un rapido test diagnostico immunocromatico (RIDT), dal campionamento della biopsia cerebrale all'interpretazione finale. Descriviamo anche ulteriori applicazioni utilizzando il dispositivo per l'analisi molecolare e la genotipizzazione virale.

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Mauti, S., Léchenne, M., Naïssengar, S., Traoré, A., Kallo, V., Kouakou, C., Couacy-Hymann, E., Gourlaouen, M., Mbilo, C., Pyana, P. P., Madaye, E., Dicko, I., Cozette, P., De Benedictis, P., Bourhy, H., Zinsstag, J., Dacheux, L. Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications. J. Vis. Exp. (160), e60008, doi:10.3791/60008 (2020).

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