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The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice
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Neuroscience
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JoVE Journal Neuroscience
The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice

The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice

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6,241 Views

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08:03 min

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September 19, 2019

DOI:

10.3791/59950-v

DOI:
10.3791/59950-v

•

08:03 min
September 19, 2019

•

1 Views
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1The Children’s Hospital, School of Medicine,Zhejiang University, 2The Institute of Translational Medicine, School of Medicine,Zhejiang University

Summary

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Here, we present a protocol to detect 5-hydroxymethylcytosine in cells and brain tissues, utilizing immunofluorescence staining and DNA dot-blot methods.

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5-hydroxymethylcytosine5hmCImmunofluorescenceDNA Dot-blotNeural Stem CellsMouse BrainEpigenetic ModificationGene ExpressionNeurological DisordersPerfusionFixationCryosectioningImmunostaining

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Zhuang, Y., Chen, J., Xu, W., Shu, Q., Li, X. The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice. J. Vis. Exp. (151), e59950, doi:10.3791/59950 (2019).

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